Addgene

Showing: 8181 - 8200 of 152169 results

Results per page:

Sort results by:

ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eSpCas9_sgAAVS1

Plasmid

#199213

Purpose

All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

AAVS1-gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

human U6

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-VGLUT2-pHluorin

Plasmid

#207469

Purpose

Lentiviral expression of VGLUT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VGLUT2 (Slc17a6 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hRAP-pEZT-BM

Plasmid

#177986

Purpose

Expression human RAP containing a C-terminal 6xHis tag in mammalian cells

Depositor

Lora Hooper

Article

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein associated protein 1 (LRPAP1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-GR

Plasmid

#215094

Purpose

Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NR3C1 (NR3C1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MscL(G22S)-c-term-cpGFP-ERexp

Plasmid

#195346

Purpose

Control plasmid for the MscL tension biosensor

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

MscL(G22S)-c-term-cpGFP-ERexp

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVd2

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbsr-JNK KTR-mCherry

Plasmid

#115493

Purpose

Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

JNK KTR-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

mCherry G229L, mCherry Y298 deleted

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23b-Casp9-His

Plasmid

#11829

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 (CASP9 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pmCherry C1 Omp25

Plasmid

#157758

Purpose

Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

SYNJ2bp (SYNJ2BP Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EF06R

Plasmid

#42940

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Human L1 element (L1RE1 Human)

Use

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

Promoter

CMV MIE, L1 5'UTR

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TNFR1-YFP

Plasmid

#111209

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TNFRSF1A (TNFRSF1A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-Ad5Knob

Plasmid

#198289

Purpose

Produces PVCs retargeted with knob domain from hAd5 virus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5Knob

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84261

Purpose

Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge