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Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
UseTagsExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 313
Plasmid#98586PurposepNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).DepositorInsertcsrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 314
Plasmid#98584PurposepCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).DepositorInsertsCsrA
csrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst2 SARAH
Plasmid#84298PurposeExpresses Mst2 SARAH domain for interaction and spectroscopy studies.DepositorInsertMst2 (STK3 Human)
UseTagsGSTExpressionBacterialMutationL443WPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Cx43-7
Plasmid#57603PurposeLocalization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCx43
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSETC PITPbeta N89F
Plasmid#58279Purposebacterial expression of PITP beta N89F mutantDepositorInsertPITP beta (PITPNB Human)
UseTagsHISExpressionBacterialMutationN89F (abolishes PI binding)PromoterT7Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GST
Plasmid#41613DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-H122Y
Plasmid#33085DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationH122YPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR S72A
Plasmid#179726PurposeLentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate releaseDepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationS72APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
Plasmid#179719PurposeLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag
Plasmid#179721PurposeLentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only