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pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

eGFP and iLID

Expression

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Promoter

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

Plasmid

#212108

Purpose

Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2

Depositor

Dieter Willbold

Article

Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

Insert

GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Human, Entacmaea quadricolor)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of both GABARAPs

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV and mCherry

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

Plasmid

#201120

Purpose

RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

TurboRFP; H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Availability

Academic Institutions and Nonprofits only

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pPRKCZ-C20-EGFP (C-PRKCZ-C20)

Plasmid

#217761

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C20 domain (aa 405-592)

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

Plasmid

#217762

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Linker GGSSGGGA plus C20 domain (aa 405-592)

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

Plasmid

#217763

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pSET-EMD-EGFP (C-SET-EMD)

Plasmid

#217764

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226)

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-SET-EMD (N-SET-EMD)

Plasmid

#217765

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226)

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-gfap

Plasmid

#75024

Purpose

Can be used to drive expression in zebrafish astrocytes.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

glial fibrillary acidic protein promoter (gfap Zebrafish)

Use

5' entry vector for multisite gateway three …

Tags

Expression

Mutation

Promoter

gfap

Availability

Academic Institutions and Nonprofits only

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TriEx-4 DHX36

Plasmid

#68368

Purpose

Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

Depositor

James Vaughn

Article

Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

Insert

DHX36 (DHX36 Human)

Use

Tags

Histidine

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV ie enhancer/promoter

Availability

Academic Institutions and Nonprofits only
pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)

Use

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Use

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
sgTP53(A)

Plasmid

#85559

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
sgTP53(R273)

Plasmid

#85561

Purpose

Expresses sgRNA targeting human TP53 around codon R273

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only