-
-
p-AAV-sh[SNCA]
Plasmid#75437Purposeknockdown alpha-synuclein in rat neuronsDepositorInsertsnca (Snca Rat)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG66
Plasmid#226751PurposeExpresses exogenous GFPDepositorInsertGFP
UseLentiviralTagssfGFPExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-AAV-sh[control]
Plasmid#75438Purposecontrol shRNA vectorDepositorInsertGFP
UseAAV and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-shEsr1
Plasmid#120720PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).DepositorInsertEsr1 shRNA (Esr1 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterMouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
UseTagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0-mC-G-CL3B
Plasmid#124975Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-mTagBFP2
Plasmid#87254PurposeLentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.DepositorInsertmTagBFP2
UseLentiviralTags6xHisExpressionMutationPromoterCaMKIIaAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoB
Plasmid#196052PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB2176
Plasmid#113395PurposeTy3-IN-GFP; alpha factor inducibleDepositorInsertTy3 with integrase-green fluorescent protein
UseTagsIN-GFPExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dyn1(K44A)-mRFP
Plasmid#55795PurposeDominant negative Dynamin 1 mutant tagged with mRFPDepositorInsertDynamin-1(K44A)-mRFP (Dnm1 Rat, Discosoma sp.)
UseTagsmRFPExpressionMammalianMutationDynamin-1(K44A)-mRFP was produced from Dynamin-1(…PromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-shEsr2
Plasmid#120722PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).DepositorInsertEsr2 (Esr2 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a1SE
Plasmid#49168PurposepHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
UseTagsmyc and pHGFP (pHIuorin)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKG204
Plasmid#63078PurposeRetroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)DepositorInsertHP1 alpha (CBX5 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationV151I mutation compared to GenBank reference sequ…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pCellFree_G03 AKT1
Plasmid#67102PurposeStandard for cell-free expression benchmarking.DepositorInsertAKT1 (AKT1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCAG-2dV-Camui (wt)
Plasmid#220366PurposeImproved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)DepositorInsertmVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
UseTagsmEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
