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Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaZ
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-FLAG-TAOK2
Plasmid#197862PurposeExpression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 S100A1
Plasmid#67048PurposeStandard for cell-free expression benchmarking.DepositorInsertS100A1 (S100A1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX:B3-α4ΔICD
Plasmid#208513PurposeddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:B1-α4ΔICD
Plasmid#208511PurposeddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:α4ΔICD-EC4-GA
Plasmid#208512PurposeddGFP-A-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Blast
Plasmid#124981PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Hph
Plasmid#124980PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Zeo
Plasmid#124979PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-cG-BSD
Plasmid#124982PurposeExpress Luc2, copGFPDepositorInsertLuc2, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Neo
Plasmid#124978PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-CMV-MARCer-Blast
Plasmid#160550Purposelabeling hypoxic cellsDepositorInsertMARCer (HIF1A Human)
UseLentiviralTagseGFP-CreExpressionMutationPromoterCMVAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TdIF2
Plasmid#196906Purposevisualization of TdIF2/ERBP as a fusion to mCherryDepositorInsertTdIF2
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald
Plasmid#203798PurposeLentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationH1047RPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Nrxn3α-AS4-
Plasmid#184332PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only