Addgene

159,111 results

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUAV_HA

Plasmid

#207273

Purpose

Mammalian expression of Influenza A virus HA protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUAV_HA (HA Influenza A virus)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-tTRKRAB

Plasmid

#12249

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis and TEV site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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ORFtag_TetR-SD_Frame1

Plasmid

#220981

Purpose

ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

Depositor

Alexander Stark

Insert

TetR repressor

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV4_PrME

Plasmid

#207266

Purpose

Mammalian expression of Dengue virus 4 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV4_PrME (POLY Dengue virus 4)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-RalA(WT)

Plasmid

#224274

Purpose

Cell transfection and expression of RalA

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Ecoli PancACe

Plasmid

#215712

Purpose

Expresses acetyl-CoA biosensor in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1-Cre-PGK-Puro

Plasmid

#108543

Purpose

Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter

Depositor

Javier Alcudia

Inserts

cre recombinase
Puromycin

Use

Tags

Expression

Mutation

Promoter

EF1apha and PGK

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Use

Tags

None

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cre

Plasmid

#34564

Purpose

Retroviral expression of Cre

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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4X PRE TK luc

Plasmid

#206159

Purpose

Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE TK luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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