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Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pcDNA3
Plasmid#18955DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
Plasmid#61591PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R273H
Plasmid#16439DepositorInsertp53 R273H (TP53 Human)
UseTagsExpressionMammalianMutationR273HPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH c-Met
Plasmid#86498PurposeMammalian expression of human c-MetDepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-SMARCA4
Plasmid#65391PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha WT
Plasmid#16390DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCh-alpha-tubulin
Plasmid#49149Purposeexpression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.DepositorInsertalpha tubulin (TUBA1B Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
458 p3E-2A-EGFP-CAAXpA
Plasmid#195968PurposeGateway p3E 2A fluorophoreDepositorInsert2A-EGFP-CAAX (membrane)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-NFIA
Plasmid#141403PurposeInducible Expression of NFIADepositorInsertNFIA (NFIA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMRX-No-HaloTag7-mGFP-LC3
Plasmid#184901PurposeStable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_a_tubulin_IRES_puro2b
Plasmid#21041DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
Plasmid#140659PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(F74G) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only