LM670
(Plasmid
#191552)
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PurposeLM670_MSG-AGGA-promoter-ccdb-TTCC-GShis
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET29b+
- Total vector size (bp) 5674
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsencodes E. coli lethal ccdb gene for selection, grow only in ccdb resistant strains such as NEBStable, or ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameccdb
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SpeciesE. Coli
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Insert Size (bp)460
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Tag
/ Fusion Protein
- HIS-tag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LM670 was a gift from David Baker (Addgene plasmid # 191552 ; http://n2t.net/addgene:191552 ; RRID:Addgene_191552) -
For your References section:
Hallucinating symmetric protein assemblies. Wicky BIM, Milles LF, Courbet A, Ragotte RJ, Dauparas J, Kinfu E, Tipps S, Kibler RD, Baek M, DiMaio F, Li X, Carter L, Kang A, Nguyen H, Bera AK, Baker D. Science. 2022 Oct 7;378(6615):56-61. doi: 10.1126/science.add1964. Epub 2022 Sep 15. 10.1126/science.add1964 PubMed 36108048
