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Showing: 8161 - 8180 of 9019 results
  1. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid
    #100989
    Purpose
    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid
    #100990
    Purpose
    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pJL30

    Plasmid
    #174064
    Purpose
    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
    Depositor
    Insert
    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. Ash1

    Plasmid
    #169711
    Purpose
    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)
    Depositor
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-500
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. RIB ONLY R3

    Plasmid
    #161511
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R3 in the RIB1 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. RIB ONLY R4

    Plasmid
    #161512
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R4 in the RIB1 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. THI ONLY pALD4 T1

    Plasmid
    #161498
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. THI ONLY pALD4 T2

    Plasmid
    #161499
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. THI ONLY pALD4 T3

    Plasmid
    #161500
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T3_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T3 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. THI ONLY pALD4 T4

    Plasmid
    #161501
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T4_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T4 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. THI ONLY pALD4 T5

    Plasmid
    #161502
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T5_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T5 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. THI ONLY pGAP T-

    Plasmid
    #161503
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. THI ONLY pGAP T1

    Plasmid
    #161504
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T1_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. THI ONLY pGAP T2

    Plasmid
    #161505
    Purpose
    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T2_gRNA_MS2
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 8161 - 8180 of 9019 results