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pcDNA3.1/NDUFS2 W61A/myc-His

Plasmid

#127500

Purpose

Expresses myc-tagged NDUFS2/W61A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 W61A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 W61A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 K62A/myc-His

Plasmid

#127501

Purpose

Expresses myc-tagged NDUFS2/K62A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 K62A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 K62A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 P63A/myc-His

Plasmid

#127502

Purpose

Expresses myc-tagged NDUFS2/P63A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 P63A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 P63A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_6ntmut

Plasmid

#73075

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_10ntmut

Plasmid

#73076

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) C49S

Plasmid

#110071

Purpose

Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Use

Tags

His6

Expression

Bacterial

Mutation

Cysteine 49 to Serine

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-646

Plasmid

#92289

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 CED 7W7A (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

dGW182 CED: aa 940-1215 with the following point …

Promoter

Ac5

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPANXN4_WT

Plasmid

#81998

Purpose

Gateway Donor vector containing SPANXN4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPANXN4 (SPANXN4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM200A_WT

Plasmid

#81878

Purpose

Gateway Donor vector containing FAM200A ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM200A (FAM200A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPATA8_WT

Plasmid

#81866

Purpose

Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPATA8 (SPATA8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SCEL_WT

Plasmid

#81870

Purpose

Gateway Donor vector containing SCEL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SCEL (SCEL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M124V; P201T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRR23B_WT

Plasmid

#81871

Purpose

Gateway Donor vector containing PRR23B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRR23B (PRR23B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF454_WT

Plasmid

#81864

Purpose

Gateway Donor vector containing ZNF454 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ZNF454 (ZNF454 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D166A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_LCE1B_WT

Plasmid

#81841

Purpose

Gateway Donor vector containing LCE1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

LCE1B (LCE1B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2G6_WT

Plasmid

#81846

Purpose

Gateway Donor vector containing OR2G6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2G6 (OR2G6 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2T33_WT

Plasmid

#81851

Purpose

Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2T33 (OR2T33 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_DCAF4L2_WT

Plasmid

#81817

Purpose

Gateway Donor vector containing DCAF4L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DCAF4L2 (DCAF4L2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_C19orf48_WT

Plasmid

#81794

Purpose

Gateway Donor vector containing C19ORF48 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C19ORF48 (C19orf48 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNK3_p.S617F

Plasmid

#81709

Purpose

Gateway Donor vector containing WNK3,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNK3 (WNK3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S617F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNK3_p.E417Q

Plasmid

#81716

Purpose

Gateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNK3 (WNK3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E417Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge