Showing: 8161 - 8180 of 16034 results
-
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-C
Plasmid#113755PurposeGateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-C
Plasmid#113757PurposeGateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Sender_plasmid2
Plasmid#140693PurposeEncodes inducible expression of RhlI (C4-HSL quorum sensing synthase)DepositorInsertRhlI (rhlI )
UseTagsssrA degradation tag (ASV)ExpressionBacterialMutationPromoterengineered lac promoter (IPTG inducible)AvailabilityAcademic Institutions and Nonprofits only -
C417-E01: attB4-polyhedrinp>His6-nostop-attB1r
Plasmid#162932PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion with AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pD-MSP1D1
Plasmid#161607PurposeBacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formationDepositorInsertD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans were replaced b…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-EEF2K_IDG-K
Plasmid#170592PurposeGateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-EEF2K (EEF2K Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailabilityAcademic Institutions and Nonprofits only -
pJH750
Plasmid#172170PurposeMicroscopy inclusion body assayDepositorInsertpCDF.3f2, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJH751
Plasmid#172171PurposeMicroscopy inclusion body assayDepositorInsertpCDF.JM10.1, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailabilityAcademic Institutions and Nonprofits only -
-
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHIT203
Plasmid#204512PurposeBacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertC04F12.1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailabilityAcademic Institutions and Nonprofits only -
pHIT517
Plasmid#204518PurposeBacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertcec-4 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailabilityAcademic Institutions and Nonprofits only
Showing: 8161 - 8180 of 16034 results