-
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorInsertssrp1 (Ssrp Fly)
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TUBG2
Plasmid#171966PurposeExpression in cell lines of the TUBG2 geneDepositorInsertTUBG2 (TUBG2 Human)
UseTagsExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…PromoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G
Plasmid#157982PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tetra-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV126
Plasmid#124250PurposeIn vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV127
Plasmid#124251PurposeIn vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV128
Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)
Plasmid#166855PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeastDepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationK683E, H685E, K686EPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV_eA3A T31A-nCas9
Plasmid#163538PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31APromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9
Plasmid#163564PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
Plasmid#160069PurposeConditionally overexpress collybistin (CB2SH3-) and mCitrineDepositorInsertARHGEF9 (Arhgef9 Rat)
UseAAVTagsHA- in CB2SH3ExpressionMutationPromoterhuman Synapsin promoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJ4
Plasmid#158785PurposePan-neuronal nuclear-localized GCaMP expression in C. elegansDepositorInsertPrab-3::nls::GCaMP6m
UseTagsNLSExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
-