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Addgene
Showing: 8061 - 8080 of 12975 results

  1. pGEM-3XP3.RFP-tan

    Plasmid
    #83474
    Purpose
    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    3XP3.RFP-tan
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    3XP3 eye-expression Promoter
    Availability
    Academic Institutions and Nonprofits only
    83474-plasmid-map-sequence-id-159168

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  2. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  3. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  14. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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Showing: 8061 - 8080 of 12975 results