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Showing: 8041 - 8060 of 9014 results

  2. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. RT-4

    Plasmid
    #220443
    Purpose
    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.
    Depositor
    Marvin Wickens
    Article
    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do
    Insert
    PUF3 (PUF3 Budding Yeast)
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220443-plasmid-map-sequence-id-436941

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  6. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL7
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219503-plasmid-map-sequence-id-435230

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  10. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  11. pRH3105

    Plasmid
    #210164
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1 (LYS1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    P194Q
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    210164-plasmid-map-sequence-id-420960

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  17. pRH3217

    Plasmid
    #210185
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1 (LYS1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    W151M
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210185-plasmid-map-sequence-id-416325

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  18. pRH3186

    Plasmid
    #210181
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1 (LYS1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    W353G
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210181-plasmid-map-sequence-id-416319

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  19. pRH3178

    Plasmid
    #210179
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1 (ADE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    G54V
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210179-plasmid-map-sequence-id-416318

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  20. pRH3135

    Plasmid
    #210166
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1 (LYS1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    I245N
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210166-plasmid-map-sequence-id-416309

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Showing: 8041 - 8060 of 9014 results