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Plasmid#190741PurposeA lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)DepositorInsertEMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)
UseLentiviralTagsMycExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…PromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-DCX-TurboID-V5-puro
Plasmid#190742PurposeA lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)DepositorInsertDCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A_no_ccDB_mUty
Plasmid#158570PurposeV5-tagged sequence of mUty cloned into entry vector for recombinationDepositorInsertMus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-BarcodeV1
Plasmid#78633PurposeGESTALT V1 integrating barcode with Puro and EGFPDepositorInsertGESTALT target V1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
HMGA2(3'UTR DEL)+GFP
Plasmid#25406DepositorInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseLentiviralTagsExpressionMammalianMutation3" UTR is absentPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 K154A
Plasmid#130886PurposeExpresses human RAB10 K154A mutant with N-terminal GFP tagDepositorInsertRAB10 (RAB10 Human)
UseLentiviralTagsGFPExpressionMammalianMutationK154APromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
TPC2(N257A)-mCherry
Plasmid#135186PurposePore-dead mutant of TPC2, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of asparagine257 to alaninePromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-G719S
Plasmid#116256PurposeLentiviral expression of EGFR G719SDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationG719SPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GSK3B gRNA (BRDN0001145264)
Plasmid#77281Purpose3rd generation lentiviral gRNA plasmid targeting human GSK3BDepositorInsertGSK3B (GSK3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgEp300#2/Cre
Plasmid#173592PurposeExpresses a Ep300-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Ep300 (Ep300 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationC124SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.FLAG.HA_NGFR
Plasmid#158294PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHSV.FLAG.HAExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-iRFP670
Plasmid#110694PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670DepositorInsertCPSF6-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationTruncation of CPSF6 isoform at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rgl2-CAAX-pcw107-V5
Plasmid#64641Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRgl2 plus C-term KRAS (Kras Mouse)
UseLentiviralTagsV5ExpressionMutationC-term of KrasPromoterPGKAvailable sinceDec. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
COPE_pLX307
Plasmid#98327PurposeLentiviral expression of COPEDepositorInsertCOPE (COPE Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-BaeI-Pgk-Cre
Plasmid#173663PurposeVector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinaseDepositorInsertBaeI site
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1374
Plasmid#143955PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF143 (ZNF143 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3338
Plasmid#144814PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only