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pcDNA6.2-YFP-CWT

Plasmid

#176689

Purpose

Fluorescent reporter for phagosomal escape of Staphylococcus aureus

Depositor

Martin Fraunholz

Article

Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

Insert

cell wall-targeting domain of Lysostaphin

Use

Tags

YFP

Expression

Mammalian

Mutation

deletion of protase domain of Lysostaphin

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_LC3A

Plasmid

#73946

Purpose

For recombinant expression of human LC3A/MAP1LC3A in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3A (MAP1LC3A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pHIV-ARF-mOrange2

Plasmid

#110731

Purpose

Mammalian Expression of p14ARF IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p14ARF IRES H2B-mOrange2 (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_PER1_WT

Plasmid

#82152

Purpose

Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PER1 (PER1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP6C_p.E180K

Plasmid

#81334

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E180K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCZ_WT

Plasmid

#82228

Purpose

Gateway Donor vector containing PRKCZ , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCZ (PRKCZ Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRIM55_WT

Plasmid

#81829

Purpose

Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRIM55 (TRIM55 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_POU2F2_WT

Plasmid

#81873

Purpose

Gateway Donor vector containing POU2F2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POU2F2 (POU2F2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WWTR1_WT

Plasmid

#82253

Purpose

Gateway Donor vector containing WWTR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WWTR1 (WWTR1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RNF212_WT

Plasmid

#81860

Purpose

Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RNF212 (RNF212 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

W4L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_WT

Plasmid

#81787

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nprot-HF_GC3opt

Plasmid

#157732

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot-HF_GC3opt (N SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

Plasmid

#174900

Purpose

amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

Insert

CRFR1 (Crhr1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

A95TAG

Promoter

EF1

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Human, Mouse)

Use

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAGEC2_WT

Plasmid

#81862

Purpose

Gateway Donor vector containing MAGEC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAGEC2 (MAGEC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13-HF_GC3opt

Plasmid

#157716

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R4_WT

Plasmid

#81859

Purpose

Gateway Donor vector containing PIK3R4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R4 (PIK3R4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T231R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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