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Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
3×Cherry Dam AID Smash
Plasmid#227374PurposeFor inducible expression and cytosolic localization of DamDepositorInsertDam DNA-adenine methyltransferase
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf cdh1-deltaCyot-GFP
Plasmid#185412PurposeGFP tagged zebrafish cdh1 lacking the cytoplasmic domain.DepositorInsertcdh1 (cdh1 Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationCytoplasmic domain deletedPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 243-450
Plasmid#24392DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 243-450.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 151-241
Plasmid#24390DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 151-241.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)
Plasmid#85038PurposeFor Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.DepositorInsertFRT-stop-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Tubulin-C-18
Plasmid#158008PurposeVisualization of tubulin in mammalian cells using mGoldDepositorInsertTubulin (TUBA1B Human)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-RHOB
Plasmid#182237PurposeExpresses RHOB GTPase with tripartite split GFP10 tagDepositorInsertras homolog family member B (RHOB Human)
UseTagsGFP10 (split-GFP tripartite)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMflET-o4
Plasmid#101314PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 1-242
Plasmid#24391DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-242.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianMutationPromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
TaRGET-ABE-C3.1 ver4.1
Plasmid#188251PurposeHypercompact adenine base editorsDepositorInsertTaRGET-ABE3.1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
1099 pcDNA GFP FKHR AAA
Plasmid#9023DepositorInsertFKHR AAA (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorInsertmCAT1 (Slc7a1 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only