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Showing: 781 - 800 of 8838 results

  2. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  3. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pEN_TT luciferase-V5

    Plasmid
    #220360
    Purpose
    Gateway entry vector for an inducible V5-tagged luciferase
    Depositor
    Kevin Janes
    Insert
    luciferase
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220360-plasmid-map-sequence-id-447352

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  14. pEN_TT CVB3 A67G

    Plasmid
    #216788
    Purpose
    Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation
    Depositor
    Kevin Janes
    Insert
    CVB3
    Use
    Entry vector for gateway cloning
    Tags
    Expression
    Mutation
    A67G in VP4
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216788-plasmid-map-sequence-id-434924

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  15. pDONR221-RBM11

    Plasmid
    #192748
    Purpose
    Gateway entry vector encoding human RBM11
    Depositor
    Summer Thyme
    Insert
    RBM11 (RBM11 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192748-plasmid-map-sequence-id-377790

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  16. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Gloria Rudenko
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59733-plasmid-map-sequence-id-93744

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  18. pDONR221-mis18a

    Plasmid
    #192747
    Purpose
    Gateway entry vector encoding zebrafish mis18a
    Depositor
    Summer Thyme
    Insert
    mis18a (si:dkey-189i22.1 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192747-plasmid-map-sequence-id-377798

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  19. pDONR221-chodl

    Plasmid
    #192738
    Purpose
    Gateway entry vector encoding zebrafish chodl
    Depositor
    Summer Thyme
    Insert
    chodl (zgc:110088 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S192I
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192738-plasmid-map-sequence-id-377797

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  20. pDONR221-ncam2

    Plasmid
    #192739
    Purpose
    Gateway entry vector encoding zebrafish ncam2
    Depositor
    Summer Thyme
    Insert
    ncam2 (ncam2 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192739-plasmid-map-sequence-id-377779

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Showing: 781 - 800 of 8838 results