Addgene

Showing: 781 - 800 of 1088 results

Results per page:

pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205548

Purpose

Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-p6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205551

Purpose

Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-I

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205552

Purpose

Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-p6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-KEIMA-REEP5

Plasmid

#201928

Purpose

PiggyBac Vector expressing KEIMA-REEP5

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

REEP5 (REEP5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-TEX264ΔLIR-GFP

Plasmid

#201930

Purpose

PiggyBac Vector expressing TEX264ΔLIR-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

F273A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-TEX264-GFP

Plasmid

#201931

Purpose

PiggyBac Vector expressing TEX264-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-FAM134C-GFP

Plasmid

#201932

Purpose

PiggyBac Vector expressing FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1067L

Plasmid

#200253

Purpose

pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

6 gRNAs targeting Doublesex, Intersex, bTublin

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_FLAG-G1 PylRS

Plasmid

#154763

Purpose

plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

Plasmid

#154770

Purpose

plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in GFP reporter, G1 PylT with A41AA and C5…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Use

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

Plasmid

#154779

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Use

Tags

LaG17

Expression

Mammalian

Mutation

204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Availability

Academic Institutions and Nonprofits only

Enlarge
pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster vasa

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

Plasmid

#214977

Purpose

Flag tagged LDS mutant LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B_mut (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

LDS mutant

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Tre-3G

Availability

Academic Institutions and Nonprofits only

Enlarge