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Showing: 781 - 800 of 1538 results

  2. pOpen-T4gene30

    Plasmid
    #165554
    Purpose
    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    T4-DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165554-plasmid-map-sequence-id-339904

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  3. pOpen-T5gene12/13

    Plasmid
    #165559
    Purpose
    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    T5 Exonuclease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165559-plasmid-map-sequence-id-339952

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  5. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  6. L0I75_tUBQ10_GG5-GG6

    Plasmid
    #219714
    Purpose
    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG5-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT terminator with added golden gate linkers
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219714-plasmid-map-sequence-id-439236

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  7. L0I89-pMUTE_GG0-1

    Plasmid
    #219708
    Purpose
    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pMUTE-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    219708-plasmid-map-sequence-id-435737

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  8. L0I74-_tUBQ10_GG4-GG6

    Plasmid
    #219713
    Purpose
    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT terminator with added golden gate linkers
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219713-plasmid-map-sequence-id-435864

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  9. L0I87-SPCHPromoter_GG0-1

    Plasmid
    #219707
    Purpose
    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219707-plasmid-map-sequence-id-435861

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  10. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219715-plasmid-map-sequence-id-435740

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  11. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  12. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  13. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

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  15. lentiGuideSLC35C1-neo

    Plasmid
    #220868
    Purpose
    encodes CRISPR sgRNA targeting human SLC3C1
    Depositor
    Jennifer Kohler
    Insert
    SLC35C1 (SLC35C1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220868-plasmid-map-sequence-id-440867

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  16. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

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  17. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  18. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

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  20. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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Showing: 781 - 800 of 1538 results