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Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only
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pMiniP
Plasmid#118049PurposeGBPart - Minimal Promoter for expression in mammalian cellsDepositorInsertminimal TATA-box promoter with low basal activity
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
phCMV-IE1
Plasmid#118048PurposeGBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cellsDepositorInsertCytomegalovirus enhancer and promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134A
Plasmid#69276PurposeGolden Gate entry vector; 4th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C
Plasmid#69284PurposeGolden Gate entry vector; 1st gRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132C
Plasmid#69285PurposeGolden Gate entry vector; 1st gRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a3
Plasmid#177875PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized the FnCas12a mutant EP16
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP670nano
Plasmid#127435PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertLAMP1-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MBP-yATOM_mCherry
Plasmid#209707PurposeMammalian expression of y-ATOM biosensor for mCherryDepositorInsertMBP-yATOM_mCherry
UseTagsMBPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF278
Plasmid#167673PurposeIntermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC15-P1B3
Plasmid#202856PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15DepositorInsertEGFP/BC15
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC11-P1A11
Plasmid#202852PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11DepositorInsertEGFP/BC11
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLuc
Plasmid#135288PurposeGBPart - GLuc Luciferase CDSDepositorInsertGaussia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrans_9xD3B_Cluc_Norm
Plasmid#68415PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.DepositorInsertCypridina Luciferase-2A-Venus YFP
UseLuciferaseTagsExpressionMammalianMutationYFP: M153T,V163A,S175G ("Venus") YFP Va…PromoterminimalCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_TOMM20-STf
Plasmid#182002PurposeCMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell linesDepositorInsertTOMM20-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCascade(Ptr)-entry(BsaI)
Plasmid#175579PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL
Plasmid#175540PurposeExpression of HaloTag9 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag9-KDEL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only