Addgene

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pVenus-PICK1-shRNA

Plasmid

#72575

Purpose

Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only
myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pCB’

Plasmid

#162703

Purpose

pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Use

Tags

Expression

Bacterial

Mutation

5513T>G (tetR E37A); 7758G>T (second tet op…

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-C1-PKCgamma-C1A

Plasmid

#21205

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-C1A (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-C1A domain. (Lab plasmid WC 0018)

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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PLK3 gRNA (BRDN0001146265)

Plasmid

#77470

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK3 (PLK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PLK3 gRNA (BRDN0001146656)

Plasmid

#77471

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK3 (PLK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PLK3 gRNA (BRDN0001145739)

Plasmid

#77472

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK3 (PLK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ro1

Plasmid

#24504

Depositor

Bruce Conklin

Insert

RASSL Ro1 (OPRK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

mutations in extracellular loop 2, making it a RA…

Promoter

Available since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Use

Tags

Expression

Mammalian

Mutation

G324D

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Use

Tags

Expression

Mammalian

Mutation

R334W

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα-EGFP

Plasmid

#181840

Purpose

GFP-tagged PKA RIα subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pShuttle AMPK gamma1

Plasmid

#15998

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pAMPK gamma1

Plasmid

#15996

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Use

Tags

His6

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only