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rat Kv3.1-HA

Plasmid

#113055

Purpose

Expresses rat Kv3.1 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

rat Kv3.1 (Kcnc1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-GRM1-IRES-CyRFP1

Plasmid

#195355

Purpose

mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

GRM1-IRES-CyRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1E KI

Plasmid

#131481

Purpose

Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mNeonGreen

Plasmid

#196864

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mNeonGreen (Nedd1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-Cdk5rap2

Plasmid

#196859

Purpose

Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Use

Tags

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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CGBE1-VRQR (pBM1192)

Plasmid

#140254

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_VRQR-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-b5 ER

Plasmid

#38274

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Cytochrome b5 (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Cyb5a Tranmembrane region from 95 to 134 amino ac…

Promoter

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE Cplx1-GFP KI

Plasmid

#131475

Purpose

Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiviral shRNA GEF-H1

Plasmid

#21477

Depositor

Richard Huganir

Article

Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
GSE22 T329A in pENTR1A (w187-1)

Plasmid

#22253

Depositor

Eric Campeau

Insert

Genetic suppressor element #22, T329A

Use

Entry vector

Tags

Expression

Mutation

Threonine 329 is mutated to Alanine (p53 nomencat…

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-NLGN2

Plasmid

#82545

Purpose

Overexpression vector for V5-NLGN2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Use

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pCR8-Magneto2.0-p2A-mCherry

Plasmid

#74334

Purpose

For gateway cloning Magneto2.0-p2A-mCherry into destination vector

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTH1, FTL, Trpv4 Rat, Human)

Use

Gateway vector

Tags

Flag and p2A-mCherry

Expression

Mutation

TRPV4: delta 760–871

Promoter

none

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mCyRFP2-CREB

Plasmid

#137008

Purpose

AAV backbone, mCyRFP2-CREB donor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mCyRFP2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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