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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Use

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-progerin

Plasmid

#17663

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-D50 lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CW3SL-EGFP

Plasmid

#61463

Purpose

AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

Depositor

Bong-Kiun Kaang

Article

Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)

Plasmid

#108292

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-GFP

Plasmid

#124770

Purpose

3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-OVA

Plasmid

#64599

Purpose

Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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phCMV-GALV-MTR

Plasmid

#163612

Purpose

Retro and Lentiviral transduction of primary human germinal center B cells

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

GaLV MTR envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-pBAD

Plasmid

#54762

Purpose

Localization: Protein Expression Vector , Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pC034 - LwCas13a-msfGFP-2A-Blast

Plasmid

#91924

Purpose

Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Vimentin-7

Plasmid

#56439

Purpose

Localization: Intermediate Filaments, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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