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Plasmid#139597Purposeexpresses KinO1 in E. coliDepositorInsertkinO1
UseTagsN terminal hexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClbN-NHis
Plasmid#51497PurposeNRPS module containing CATE domains with N-terminal 6His tagDepositorInsertClbN
UseTags6x His tag and T7 tagExpressionBacterialMutationcontains aa30-1094 of NP_754347.1PromoterT7Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ZnF-UBPdel
Plasmid#90299Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationZnF-UBP deletedPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28-Rsn2
Plasmid#61686Purposebacterial expression of ranaspumin 2 as a His-tagged fusion proteinDepositorInsertRanaspumin-2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaM
Plasmid#224428PurposeExpress AzaM in E. coliDepositorInsertazaM
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaC
Plasmid#224424PurposeExpress AzaC in E. coliDepositorInsertazaC
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaD
Plasmid#224425PurposeExpress AzaD in E. coliDepositorInsertazaD
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaB
Plasmid#224423PurposeExpress AzaB in E. coliDepositorInsertazaB
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaG
Plasmid#224427PurposeExpress AzaG in E. coliDepositorInsertazaG
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaE
Plasmid#224426PurposeExpress AzaE in E. coliDepositorInsertazaE
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80-421)
Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
M59 pPET28a-TEV-human beta cat domains R1-C
Plasmid#17200DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsHISExpressionBacterialMutationdomains R1CPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M57 pPET28a-TEV-full-length human beta catenin
Plasmid#17198DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsHISExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M58 pPET28b-TEV-full-length zebrafish beta catenin
Plasmid#17199DepositorInsertbeta catenin (ctnnb1 Zebrafish)
UseTagsHISExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80-142)
Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only