Skip to main content
Addgene
Showing: 61 - 80 of 153 results

  9. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid
    #182527
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    7 Copies of TetO Box
    Use
    Tags
    4XFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182527-plasmid-map-sequence-id-358175

    Enlarge

  11. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

    Enlarge

  17. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193314
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193314-plasmid-map-sequence-id-382366

    Enlarge

  18. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

    Enlarge

  19. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193316
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193316-plasmid-map-sequence-id-382501

    Enlarge

  20. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

    Enlarge

Showing: 61 - 80 of 153 results