Addgene

We narrowed to 20,410 results for: crispr

Showing: 61 - 80 of 20410 results

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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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epiCRISPRe

Plasmid

#135962

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-6D

Plasmid

#149387

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

6bp deleted downstream of the IHFprox site

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-5D

Plasmid

#149388

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

5bp deleted downstream of the IHFprox site

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+5D

Plasmid

#149390

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

5bp inserted downstream of the IHFprox site

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Opt

Plasmid

#149395

Purpose

P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHFprox site is optimized at key positions needed…

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Mut

Plasmid

#149396

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHFprox site is mutated at key positions needed f…

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_motif_scram

Plasmid

#149397

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

Upstream motif recognized by Cas1-2/3 during spac…

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Article

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Use

Tags

Expression

Mutation

Promoter

araBAD

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRCreb5gRNA

Plasmid

#195021

Purpose

Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Creb5 gRNA (CREB5 Bovine)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_2

Plasmid

#195133

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 2 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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