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Plasmid#99394PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1DepositorInsertBRCA1 (BRCA1 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOM20*-SBP-GFP
Plasmid#120173PurposeExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)DepositorInsertMitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
UseTagsSBP and eGFPExpressionMammalianMutationTruncated TOM20: 1-30 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEX3*-SBP-GFP
Plasmid#120174PurposeExpresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)DepositorInsertPeroxisomal biogenesis factor 3 (PEX3 Human)
UseTagsSBP and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_TPV-CXCL9-mCherry-SBP
Plasmid#170677PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.DepositorInsertsUseTagsSBP and mCherryExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-USP7
Plasmid#99393PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7DepositorInsertUSP7 (USP7 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorInsertVAMP associated protein A (VAPA Human)
UseTagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINTO-NSA::mEzh2
Plasmid#61725PurposeExpression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)DepositorInsertEnhancer of zeste homolog 2 (Ezh2 Mouse)
UseTagsmSA (monomeric streptavidin)ExpressionMammalianMutationPromoterT-REx (CMV + 2xTetO2)Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINTO-NSA::hSNRNP70
Plasmid#61726PurposeExpression of human SNRNP70 fused to monomeric streptavidin (N-terminus)DepositorInsertSNRP70 (SNRNP70 Human)
UseTagsmSA (monomeric streptavidin)ExpressionMammalianMutationTwo silent mutations to ensure restriction site c…PromoterT-REx (CMV + 2xTetO2)Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-CXCL9-mCherry-SBP
Plasmid#170678PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsUseTagsSBP and mCherryExpressionMammalianMutationChanged threonine 23 to glutamate (T23E)PromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c043
Plasmid#139766PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationContains only amino acids E41- D225PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c027
Plasmid#139764PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c044
Plasmid#139767PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationG177D; contains only amino acids E41- D225PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-SBP
Plasmid#67173PurposeCRISPaint universal donor for streptavidin binding peptide fusion taggingDepositorInsertSBP
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCS2+Cas9-mSA
Plasmid#103882PurposeCas9 mSA for in vitro transcription for gene editing in Mouse EmbryosDepositorInsertCAS9-MSA
UseCRISPRTagsMSA (monomeric streptavidinExpressionMutationnonePromoterSP6Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinExpressionMutationnonePromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
STR-KDEL_PSAP-SBP-mCherry
Plasmid#180440PurposeCo-expresses (via IRES) streptavidin-KDEL and Prosaposin-SBP-mCherryDepositorInsertProsaposin (PSAP )
UseTagsSBP-mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-mCherry
Plasmid#65301Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
VSVGwt-SBP-EGFP_puromycin
Plasmid#65302PurposeVSVG reporter only (no hook) (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only