Skip to main content
Addgene
Showing: 61 - 80 of 7073 results

  1. pAC026

    Plasmid
    #227598
    Purpose
    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)
    Depositor
    Brittany Adamson
    Article
    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    227598-plasmid-map-sequence-id-454348

    Enlarge

  2. pACT5FVMF(f)

    Plasmid
    #110549
    Purpose
    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
    Depositor
    David Russell
    Article
    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10
    Insert
    EGFP, FV LTRs
    Use
    Qpcr control for titering foamy virus
    Tags
    Expression
    Mutation
    Promoter
    promoter: MLV LTR
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110549-plasmid-map-sequence-id-212295

    Enlarge

  10. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

    Enlarge

  13. pACλCI

    Plasmid
    #53730
    Purpose
    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    bacteriophage lambda CI protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac UV5
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53730-plasmid-map-sequence-id-83908

    Enlarge

  15. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

    Enlarge

  18. pAcCas12nHS_RV6_empty

    Plasmid
    #203811
    Purpose
    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.
    Depositor
    Quanjiang Ji
    Article
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Insert
    AcCas12nHS_RV6
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203811-plasmid-map-sequence-id-405075

    Enlarge

Showing: 61 - 80 of 7073 results