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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Synthetic, Mouse)
3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

Plasmid

#34631

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

rat Syntaxin1a (Stx1a Rat)

Use

Tags

meGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn–GFP

Plasmid

#35956

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Clavesin1 (pEGFP-C1-Sec14a)

Plasmid

#47421

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin2 (pEGFP-C1-Sec14b)

Plasmid

#47420

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCSC-GFP-NES-EGFP

Plasmid

#221810

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

GFP-NES-EGFP

Use

Lentiviral

Tags

NES (internal tag)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

Plasmid

#219536

Purpose

a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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