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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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GFP-Clavesin2 (pEGFP-C1-Sec14b)

Plasmid

#47420

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin1 (pEGFP-C1-Sec14a)

Plasmid

#47421

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha1-EGFP

Plasmid

#126463

Purpose

Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

Insert

Myelin oligodendrocyte glycoprotein (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GFP-fGFP

Use

AAV

Tags

Expression

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GFP10-ZIP-GFP11

Plasmid

#182243

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP-11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCSC-GFP-NES-EGFP

Plasmid

#221810

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

GFP-NES-EGFP

Use

Lentiviral

Tags

NES (internal tag)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge