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Plasmid#85614PurposeInducible dCas9 integration vector for Bacillus subtilisDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterPxylA (B. megaterium)Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Gate2 mazEF
Plasmid#117117PurposeDestination vector with mazEF toxin antitoxin systemDepositorTypeEmpty backboneUseBackbone for golden gate assembly for gene target…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW014-2
Plasmid#85612PurposeMulti-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilisDepositorInsertugtP-gRNA.P395T
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETB BS2mT4L1
Plasmid#141203PurposeChimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.DepositorInsertBS2m-T4L1
UseTagsExpressionBacterialMutationE11QPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-KPS12-Pman
Plasmid#133832PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc
Plasmid#129105PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone productionDepositorInsertBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Tev-Avi-tag
Plasmid#117128PurposeC-terminal Tev-Avi tag TagDepositorInsertTev Avi-tag
UseGolden gate donor vector for gene targeting in ba…TagsTev Avi-TagExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Tev-Halo tag
Plasmid#117124PurposeC-terminal Tev Halo-TagDepositorInsertTev-Halo-tag Tag
UseGolden gate donor vector for gene targeting in ba…TagsTev-Halo-TagExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 HA6-Tag
Plasmid#117126PurposeC-terminal HA-tagDepositorInsert6xHA Tag
UseGolden gate donor vector for gene targeting in ba…Tags6xHA tagExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Pk6-tag
Plasmid#117127PurposeC-terminal Pk6-tagDepositorInsert6xPK tag
UseGolden gate donor vector for gene targeting in ba…Tags6xPK tagExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorTagsExpressionMutationPromoterConstitutive tracrRNAAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSWEET-bgaB
Plasmid#210272PurposeXylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.DepositorInsertbgaB
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_155 [pIM13 ORI]
Plasmid#210677PurposeLevel 0 partDepositorInsertpIM13 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ205
Plasmid#104586PurposeExpress SNAP-6His-CBDDepositorInsertSNAP-6His-CBD
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRBBm34
Plasmid#48114PurposeExpression of the gfp gene under control of the native xylose inducible promoter of Bacillus megateriumDepositorInsertgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
UseTags6xHis and Bacillus circulans xylanaseExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only