Showing: 61 - 80 of 134 results
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Plasmid#114082PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA_221
Plasmid#169142PurposeConstitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.DepositorInsertEGFP , EGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA_112
Plasmid#136475PurposeLentiviral expression of AsCas12aDepositorInsertAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0028
Plasmid#117540PurposeLevel1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (AsCas12a)
UseTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCE048-SiT-Cas12a
Plasmid#128124PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCE059-SiT-Cas12a-[Activ]
Plasmid#128136PurposeExpress both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Activ]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI108-SiT-Cas12a-[Cond]
Plasmid#128407PurposeConditionally express both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0014
Plasmid#117550PurposeLevel 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1GG
Plasmid#131011PurposeBackbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of AsCas12a
GFP expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA_174
Plasmid#136476PurposeLentiviral expression of EnAsCas12aDepositorInsertEnAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pRDA52_Lenti.puro.U6_modified DR
Plasmid#196723PurposeFor cloning pairs of AsCas12a guides with two DRs that are as different as possible.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralTagsExpressionMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ220-Ultra
Plasmid#176889PurposeGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoterDepositorInsertAsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB22.SV40.Zeo
Plasmid#135692PurposeLentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selectionDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 134 results