...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

... many Cas12a orthologs, but the most common is AsCas12a (from Acidaminococcus sp. ). The popularity can...popular being EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR...Huang, A., & Doench, J. G. (2020). Optimization of AsCas12a for combinatorial genetic screens in human cells...

... many Cas12a orthologs, but the most common is AsCas12a (from Acidaminococcus sp. ). The popularity can...popular being EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR...Huang, A., & Doench, J. G. (2020). Optimization of AsCas12a for combinatorial genetic screens in human cells...

...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...