Showing: 151 - 188 of 188 results
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Plasmid#86873PurposeTo express GRB2 with a GFP tag at C terminus.DepositorInsertGRB2 (GRB2 Human)
UseTagsGFPExpressionMammalianMutationnonePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJD427
Plasmid#154371PurposeExpresses FKBP12-XB and SspBcore-FRB from constitutive promotersDepositorInsertproB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSP618
Plasmid#169235PurposeSpear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adaptersDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Pmyo3::tag:sl1(G16C)
Plasmid#79003PurposeExpression of exogenous SL1 in C. elegans muscleInsertsls-1.2
UseTagsillumina-adapterExpressionWormMutationG16CPromotermyo-3AvailabilityAcademic Institutions and Nonprofits only -
pSW609 - N001
Plasmid#115993Purposeintermediate vector for GreenGate cloning method, contains modules from A-FDepositorInsertH-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseUnspecifiedTagsHDEL and SP(ER)ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA
Plasmid#214614Purposeadapter for RTK split TEV recruitment assaysDepositorInsert3xSH2 domains of GRB2-CTEV (GRB2 Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Lhx2
Plasmid#61537PurposeExpresses Lhx2 in mammalian cellsDepositorInsertLhx2 (Lhx2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos TRIF Flag
Plasmid#41550DepositorInsertTrif (TICAM1 Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::tag:SL1(G20A)
Plasmid#81114PurposeExpression of exogenous SL1(G20A) in C. elegans muscleDepositorInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG20APromoterpromoter of myo-3AvailabilityAcademic Institutions and Nonprofits only -
Pu2-3::tag::sl1(G16C)
Plasmid#79002PurposeExpression of exogenous SL1 in multiple tissues of C. elegansInsertsls-1.2
UseTagsillumina-adapterExpressionWormMutationG16CPromoterF08G2.17AvailabilityAcademic Institutions and Nonprofits only -
BICDN-CIBN
Plasmid#102253PurposeExpresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)DepositorInsertBICDN-CIB1 (CIB1 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GFP-BICDN-CIBN
Plasmid#102254PurposeExpresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)DepositorInsertGFP-BICDN-CIB1 (CIB1 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-flex-TVA
Plasmid#69618PurposeExpress recepter TVA for envA with AAVDepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
UseAAV and Cre/LoxTagsFLAGExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS
Plasmid#98566PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein
UseTagsExpressionBacterialMutationIn a synthetic operon downstream of UBP1 and Remo…PromoterSame as UBP1 (operon) and Tet promoter (aTc induc…AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)
Plasmid#169166PurposeBaculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cellsDepositorInsert3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7 (SARS-CoV-2)
Plasmid#169178PurposeBaculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cellsDepositorInsertnsp7 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1c_nsp8 (SARS-CoV-2)
Plasmid#169179PurposeBaculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)
Plasmid#169182PurposeBaculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLIB_GST-nsp13 (SARS-CoV-2)
Plasmid#169190PurposeBaculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cellsDepositorInsertGST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsGSTExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
UseTagsExpressionBacterialMutationContains the V65I mutation that increases discrim…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169162PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cellsDepositorInsertnsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
UseTags6His-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)
Plasmid#169163PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cellsDepositorInsert3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169164PurposeBaculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cellsDepositorInsertnsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
UseTags6His-3xFlag (nsp10 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)
Plasmid#169180PurposeBaculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6 (internal tag linking nsp7 and nsp8): nsp7-H…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169181PurposeBaculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsNo purification tag and nsp7-nsp8 fusion protein …ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)
Plasmid#169183PurposeBaculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cellsDepositorInsertnsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
UseTags3xFlag (nsp12 C-terminus) and His6 (as internal t…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169184PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)
Plasmid#169185PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsHis6-3xFlag (nsp12 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)
Plasmid#169177PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cellsDepositorInsert3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
UseTags3xFlag-His6 (nsp5-cleavable)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)
Plasmid#169194PurposeBaculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cellsDepositorInsert3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorUseTagsGST-mCherryExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 151 - 188 of 188 results