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Showing: 151 - 200 of 540 results

  1. pNY-347

    Plasmid
    #231047
    Purpose
    Used for GFP staining
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to mouse Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231047-plasmid-map-sequence-id-457176

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  2. pPMS-2397

    Plasmid
    #231044
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to chicken Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    8xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231044-plasmid-map-sequence-id-457178

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  3. pNY-346

    Plasmid
    #231046
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to human Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231046-plasmid-map-sequence-id-458329

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  5. pEGFP-C1-FLAG

    Plasmid
    #46956
    Purpose
    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46956-plasmid-map-sequence-id-352459

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  6. pMVA

    Plasmid
    #121149
    Purpose
    This plamid was used for improving the production of DMAPP (IPP) in E.coli
    Depositor
    Minjuan Xu
    Article
    He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21
    Insert
    MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    121149-plasmid-map-sequence-id-246104

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  8. MCP-EGFP-PiggyBac

    Plasmid
    #190003
    Purpose
    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.
    Depositor
    Tineke Lenstra
    Article
    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi
    Insert
    MCP-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    190003-plasmid-map-sequence-id-375799

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  20. MZB215

    Plasmid
    #221767
    Purpose
    Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag
    Depositor
    Richard Baker
    Insert
    Arf6 (ARF6 Human)
    Use
    Tags
    CPD-10xHis
    Expression
    Bacterial
    Mutation
    Q67L
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221767-plasmid-map-sequence-id-455729

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  21. MZB218

    Plasmid
    #221769
    Purpose
    Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag
    Depositor
    Richard Baker
    Insert
    Arf4 (ARF4 Human)
    Use
    Tags
    CPD-10xHis
    Expression
    Bacterial
    Mutation
    Q71L
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221769-plasmid-map-sequence-id-456029

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  22. MZB219

    Plasmid
    #221770
    Purpose
    Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag
    Depositor
    Richard Baker
    Insert
    Arf5 (ARF5 Human)
    Use
    Tags
    CPD-10xHis
    Expression
    Bacterial
    Mutation
    Q71L
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221770-plasmid-map-sequence-id-455289

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  25. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TET
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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  33. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  39. MZB220

    Plasmid
    #221771
    Purpose
    Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag
    Depositor
    Richard Baker
    Insert
    Arl1 (ARL1 Human)
    Use
    Tags
    CPD
    Expression
    Bacterial
    Mutation
    Q71L
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221771-plasmid-map-sequence-id-455287

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  40. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Use
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221772-plasmid-map-sequence-id-455976

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Showing: 151 - 200 of 540 results