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We narrowed to 149 results for: crk

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mCherry-CCHD (p130Cas)

Plasmid

#145133

Purpose

Expresses CCH domain from p130Cas with an mCherry tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-PIP36

Plasmid

#196774

Purpose

Contains DORN1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

DOES NOT RESPOND TO NUCLEOTIDES 1 (LecRK-I.9 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-DGCR8(+6)

Plasmid

#171541

Purpose

Encodes mutant human DGCR8 protein

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Use

Tags

Expression

Mammalian

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CCHD (p130Cas)

Plasmid

#145132

Purpose

Expresses CCH domain from p130Cas with an EGFP tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag p38 alpha (agf)

Plasmid

#20816

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-DGCR8-2xdsRBD(+6)

Plasmid

#171542

Purpose

Encodes a DGCR8 fragment extending from E502 to the C-terminus

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pB6-Cdk6-T2A-td-sfGFP-FNF-TK

Plasmid

#134321

Purpose

B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Cdk6 (Cdk6 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tandem dimer-superfolder…

Expression

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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PCDNA3 Flag p38 alpha (agf)

Plasmid

#20352

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCAR1-1/1

Plasmid

#91418

Purpose

Protein expression and purification of human SH3 domain construct BCAR1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BCAR1-1/1 (BCAR1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pUC19_GNB1L_dTAG

Plasmid

#224378

Purpose

Generates GNB1L dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

dTAG

Expression

Bacterial

Mutation

Synonymous mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146337)

Plasmid

#75707

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146285)

Plasmid

#75708

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK20 gRNA (BRDN0001147842)

Plasmid

#75709

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK20 gRNA (BRDN0001144911)

Plasmid

#75706

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/T0-EGFP-GNB1L

Plasmid

#222621

Purpose

Mammalian expression vector of flag-tagged GNB1L

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

GNB1L (GNB1L Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

Plasmid

#52092

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge