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Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
UseTagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha
Plasmid#179713PurposeLentiviral expression of mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn spCas9
Plasmid#179725Purposehuman synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITIDepositorInsertspCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP mCherry
Plasmid#45233PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD INCENP-Δcen mCherry
UseTagsDNA binding domain of CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A
Plasmid#195707PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B
Plasmid#195708PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB
Plasmid#195709PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE
Plasmid#162380PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE
Plasmid#162378PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE
Plasmid#104492PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
Plasmid#104496PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
Plasmid#104493PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV Retrograde and AAV1InsertjGCaMP7b
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE
Plasmid#104497PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorInsertjGCaMP7b
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Plasmid#100842PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40
Plasmid#100835PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
Plasmid#217676PurposeAAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.DepositorInsertGtACR2-P2A-Voltron2ST
UseAAVTagsSoma targeting sequence (Kv2.1) on Voltron2 gene …ExpressionMammalianMutationPromoterEF‐1αAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Plasmid#100845PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Plasmid#100833PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40
Plasmid#100838PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40
Plasmid#100839PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG-DO-TC66T-mCherry
Plasmid#175178PurposeCre-off TVA-mCherry fusion protein with 66T mutationDepositorInsertTVA
UseAAVTagsmCherryExpressionMutationE66TPromoterCAGAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PP-HaloTag-SYT7alpha
Plasmid#179714PurposeLentiviral expression of mouse Syt7 with N terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD
Plasmid#179720PurposeLentiviral expression of plasma membrane-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR S72A
Plasmid#179726PurposeLentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate releaseDepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationS72APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pBS cSugarCrisp (CT#708)
Plasmid#13956DepositorInsertSugarCrisp (PI15 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS hSugarCrisp (CT#592)
Plasmid#13966DepositorInsertSugarCrisp (PI15 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt2
Plasmid#184503PurposeMammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 2 (Syt2 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt7
Plasmid#184508PurposeMammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 7 (Syt7 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only