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pET_SZ1-RGG-RGG

Plasmid

#124942

Purpose

Expression of SZ1-RGG-RGG

Depositor

Benjamin Schuster, Matthew Good, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Use

Tags

Expression

Worm

Mutation

Promoter

tag-168

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Plasmid

#159807

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pXF121

Plasmid

#139028

Purpose

MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

short mex-5 promoter (mex-5 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH54

Plasmid

#139052

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tbb-2 3'UTR (tbb-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF88

Plasmid

#139039

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (includes STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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ChrII_ttTi5605_loxP_myo2_neoR

Plasmid

#194056

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ttTi5605

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ChrV_oxTi365_loxP_myo2_neoR

Plasmid

#194057

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

oxTi365

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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KG#908

Plasmid

#110914

Purpose

Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans

Depositor

Kenneth Miller

Inserts

rab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrV_oxTi365.gRNA

Plasmid

#194052

Purpose

sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrV_oxTi365 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJRK261

Plasmid

#173754

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Article

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Use

Tags

Expression

Worm

Mutation

Promoter

rps-27 promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Use

Tags

Punc-25 and mcherry

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJL43.1 - Pglh-2::MosTase::glh-2utr

Plasmid

#19332

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pglh-2::Mos1 transposase

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Use

Tags

Expression

Worm

Mutation

Promoter

tag-168

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJG103

Plasmid

#158533

Purpose

wrmScarlet11 x3 tandem repeats

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet11 x3 tandem repeats

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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