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Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179120PurposeExpresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertcontrol sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179119PurposeExpresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertEzr sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179117PurposeExpresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertFermt2 sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
Plasmid#112496PurposeExpresses channel rhodopsin in astrocytesDepositorInsertChR2(H134R)
UseAAVTagsmCherryExpressionMutationH134RPromoterGfaABC1DAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA
Plasmid#219809PurposeSecreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labelingDepositorInsertGfaABC1D
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAExpressionMutationPromotergfaABC1D (astrocyte specific)Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6s wpre Pzac2.1
Plasmid#74150Purposecalcium sensorDepositorInsertGCaMP6s
UseAAVTagsH2BExpressionMutationPromoterSynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1
Plasmid#74144PurposeCalcium sensorDepositorInsertGCaMP6f
UseAAVTagsH2BExpressionMutationPromotersynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-PLAGL1
Plasmid#61542PurposeExpresses PLAGL1 in mammalian cellsDepositorInsertPLAGL1 (PLAGL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1054
Plasmid#142720PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPLAGL1 (PLAGL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1055
Plasmid#143462PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPLAGL1 (PLAGL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-RPB1aAmr
Plasmid#75284PurposeExpresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cellsDepositorInsertPOLR2A (POLR2A Human)
UseTagsEYFPExpressionMammalianMutationN792D alpha-amanitin–resistantPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
UseTags6X HisExpressionBacterialMutationE602DPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
UseTags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF1-VP16
Plasmid#51426PurposeArtificial transcription factor with VP16DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAF1-VP64
Plasmid#51427PurposeArtificial transcription factor with VP64DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAF1
Plasmid#51425PurposeArtificial transcription factor with no activator domainDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH1v1
Plasmid#60244PurposegRNA expression by the H1 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only