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pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196075

Purpose

Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196077

Purpose

Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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E42_control_NGFR

Plasmid

#189800

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Nat Immunol. 2018 Dec;19(12):1427-1440.

Insert

Luciferase gRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

Plasmid

#149427

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#1

Plasmid

#174323

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTK73_htz-1

Plasmid

#174546

Purpose

htz-1 targeting gRNA expression

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

htz-1 targeting gRNA

Use

Tags

Expression

Worm

Mutation

Promoter

Ce-U6 promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Nectin-2

Plasmid

#170295

Purpose

A knock-out vector for the mouse Nectin-2

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Nectin-2 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Necl-5

Plasmid

#170294

Purpose

A knock-out vector for the mouse Necl-5

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Necl-5 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZS160

Plasmid

#134238

Purpose

Lentiviral plasmid expressing sgRNA against HJURP

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human HJURP sgRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN158

Plasmid

#91687

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN159

Plasmid

#91688

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN156

Plasmid

#91685

Purpose

Express sgRNA targeting human ZNF804A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZNF804A (ZNF804A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN157

Plasmid

#91686

Purpose

Express sgRNA targeting human ZNF804A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZNF804A (ZNF804A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN118

Plasmid

#91645

Purpose

Express sgRNA targeting human MPP6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MPP6 (PALS2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN119

Plasmid

#91646

Purpose

Express sgRNA targeting human MPP6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MPP6 (PALS2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN276

Plasmid

#91647

Purpose

Express sgRNA targeting human PLCH2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PLCH2 (PLCH2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN277

Plasmid

#91648

Purpose

Express sgRNA targeting human PLCH2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PLCH2 (PLCH2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN135

Plasmid

#91649

Purpose

Express sgRNA targeting human SATB2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SATB2 (SATB2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN125

Plasmid

#91650

Purpose

Express sgRNA targeting human PPP1R16B

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PPP1R16B (PPP1R16B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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