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pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

Plasmid

#41006

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00550

Plasmid

#39645

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12245 (gcm Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pPgiCas13b-gRNA-NT

Plasmid

#184835

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag

Plasmid

#41013

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

changed amino acids 1189-1192 to Alanine

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

Plasmid

#41012

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

changed amino acids 1185-1188 to Alanine

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

Plasmid

#41011

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

changed amino acids 1183-1186 to Alanine

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
Ii-Str_ssSBP-EGFP

Plasmid

#65277

Purpose

to synchronize transport of secretory soluble GFP from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

EGFP

Use

Tags

IL-2 signal sequence and Streptavidin Binding Pr…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_SBP-EGFP-GPI

Plasmid

#65296

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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hMSH2 Clone E3 (full-length)

Plasmid

#16453

Depositor

Bert Vogelstein

Article

Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

Insert

MSH2 (MSH2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMH48

Plasmid

#131861

Purpose

Produces holo-Cph1* in E. coli

Depositor

Wilfried Weber

Article

Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

Inserts

Cph1(1-514,Y263F)-RGD-His6-Cys
HO1
PcyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter-1 and T7 promoter-2

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR; Gateway destination

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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mCD4 e/p Sal-(hCD2)

Plasmid

#24070

Depositor

Dan Littman

Article

Sawada et al Cell. 1994 Jun 17. 77(6):917-29.

Insert

CD2 (CD2 Human)

Use

Tags

mCD 4 promoter

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-NHERF1-EB

Plasmid

#28297

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (Nherf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains only the EB domain aa 262-355 (deletion …

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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