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Plasmid#157697Purposemammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt
Plasmid#157700Purposemammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp8_GC3opt
Plasmid#157698Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBOB-N-Flag-Snrnp40-skywarp
Plasmid#134250PurposeLentivector encoding Flag-tagged skywarp form of Snrnp40 (missing exon 5)DepositorInsertSnrp40 (Snrnp40 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationdeleted amino acids 179-219PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG-WPRE
Plasmid#127869PurposepAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoterDepositorInsertNos1 (Nos1 Mouse)
UseAAVTags3xFLAGExpressionMutationAmino acids 1-133 onlyPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _2-33/myc-His
Plasmid#127492PurposeExpresses myc-tagged _MTS NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _2-33 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 _2-33PromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _2-78/myc-His
Plasmid#127493PurposeExpresses myc-tagged _2-78 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _2-78 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 _2-78PromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-547-782
Plasmid#118376PurposeGateway compatible donor vector with KAT14-547-782 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 547-782)PromoterAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1-C
Plasmid#109160PurposeEncodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-1-546
Plasmid#118377PurposeGateway compatible donor vector with KAT14-1-546 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 1-546)PromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 mSesn1 (1189)
Plasmid#66917Purposebacterial expression of mouse Sesn1DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cx32R220stop
Plasmid#78151PurposeMammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNADepositorInsertConnexin 32 (GJB1 Human)
UseTagsEGFPExpressionMammalianMutationOnly contains first 219 residuesPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 - mut 3'UTR
Plasmid#97161PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationmiR-722 Binding MutantPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-660
Plasmid#92259PurposePlasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-665
Plasmid#92254PurposePlasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
csd6_17-330/pET28b(+) N-tag
Plasmid#71353Purposecsd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)DepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_E110A/pET28b(+) N-tag
Plasmid#71354Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:E110ADepositorInsertHP0518 (HP0518 changed Glutamic acid 110 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y133A/pET28b(+) N-tag
Plasmid#71356Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y133ADepositorInsertHP0518 (HP0518 changed Tyrosine 133 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only