pSi-check2-hRac2 - mut 3'UTR
(Plasmid
#97161)
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PurposeFor dual luciferase report assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePsi-check2
- Backbone size w/o insert (bp) 6250
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAC 3'UTR
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SpeciesH. sapiens (human)
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Insert Size (bp)780
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MutationmiR-722 Binding Mutant
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSi-check2-hRac2 - mut 3'UTR was a gift from Qing Deng (Addgene plasmid # 97161 ; http://n2t.net/addgene:97161 ; RRID:Addgene_97161) -
For your References section:
Overexpression of microRNA-722 fine-tunes neutrophilic inflammation through inhibiting Rac2 in zebrafish. Hsu AY, Wang D, Gurol T, Zhou W, Zhu X, Lu HY, Deng Q. Dis Model Mech. 2017 Sep 27. pii: dmm.030791. doi: 10.1242/dmm.030791. 10.1242/dmm.030791 PubMed 28954734