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Plasmid#82217PurposeGateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.DepositorInsertSMAD5 (SMAD5 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-mut (super repressor)
Plasmid#15264DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationSerine 32 to Alanine, Serine 36 to AlaninePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-AnillinFL
Plasmid#187271PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationPromoterU6, CMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK
Plasmid#58906Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removedDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationall 15 Ser/Thr Cdk acceptor sites changed to Ala,…PromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
UseTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_YTHDC1_WT
Plasmid#81799PurposeGateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.DepositorInsertYTHDC1 (YTHDC1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAP1-1
Plasmid#125837PurposeKO BAP1 geneDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGmCherry-gRNA
Plasmid#87110PurposegRNA cloning vector with CAGmCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2, transient)
Plasmid#197589PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PPP6C_WT
Plasmid#81811PurposeGateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.DepositorInsertPPP6C (PPP6C Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-c-Myc-Ubc-rtTA
Plasmid#198190PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthC-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-GSX2
Plasmid#96964PurposeDox-inducible expression of Gsx2 in mammalian cellsDepositorInsertGSX2 (Gsx2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9_sgRNA_0
Plasmid#70763Purposeexpresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicatingDepositorInsertsU6 promoter
cas9
tnos terminator
UseSelf-replicating in ustilago maydis, conferring c…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU6 from Ustilago maydis and synthetic otef promot…Available sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-h-SNRNP40
Plasmid#134254PurposeLentivector encoding Flag-tagged human SNRNP40DepositorInsertSNRP40 (SNRNP40 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertHF-BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorInsertExonuclease 1b D173A (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7 (hspb7 Zebrafish)
UseTagsHalo-TEVExpressionMammalianMutationPromoterSP6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_M18
Plasmid#82356PurposeExpression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLCA.66/2272
Plasmid#22733DepositorInsertLox66-pU(delta)TK-EM7Neo-Lox2272
UseCre/Lox; Bac recombineeringTagsExpressionMutationThis vector was designed for assembling gene targ…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only