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Plasmid#35017DepositorInsertSRC (SRC Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIb'a' tagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmeTagsExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EYFP
Plasmid#167906PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-AP3
Plasmid#190826PurposeFor mammalian expression of AP3, the RNA aptamer of GFP. Expression is driven by a U6 promoter.DepositorInsertAP3 (an RNA aptamer of GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBB-II-I-GFP gamma isoform
Plasmid#22148DepositorInsertTFII-I (GTF2I Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EBFP2
Plasmid#167857PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf9c
Plasmid#158379PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf9c
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046959
Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
px458_2A_GFP_sgRNA_NEMF
Plasmid#127126DepositorInsertgRNA NEMF (NEMF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046879
Plasmid#149238PurposeBacterial expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
VYAIFIFQL_epitope
Plasmid#165018PurposeExpression of CMV US20 (29-37) fused to IL-2 signal sequenceDepositorInsertCMV US20 (29-37)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PTD004
Plasmid#34798DepositorInsertGTP-binding protein PTD004 (OLA1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046942
Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits