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pBabe_puro_DEST_Flag_MDS1

Plasmid

#45258

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MDS1 (MECOM, MDS1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCMVblast_SLC38A2_wt

Plasmid

#156180

Purpose

CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA se…

Promoter

CMV

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNK3_p.V488A

Plasmid

#81723

Purpose

Gateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNK3 (WNK3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V488A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70 promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1)

Plasmid

#191341

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GV

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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InsRA-TagBFP

Plasmid

#79791

Purpose

Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Use

Tags

TagBFP extracellular

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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peSCBE3-NG

Plasmid

#218163

Purpose

This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

escbe3-NG
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Tags

Expression

Bacterial

Mutation

Mutation in the portion of deaminase hAPOBEC3A : …

Promoter

rpsL promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetics expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-deltaNp53:WTp53

Plasmid

#49244

Purpose

expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN and p53 WT (TP53 Human)

Use

Tags

His and IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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p2XStrep-ATIC

Plasmid

#125685

Purpose

expresses human ATIC protein with an N-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ATIC (ATIC Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHIV-BRAFV600E-mOrange2

Plasmid

#110732

Purpose

Mammalian Expression of BRAFV600E IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRAFV600E IRES H2B-mOrange2 (BRAF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

BRAF V600E

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-WT

Plasmid

#87926

Purpose

Expresses IDH2 (wild-type) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11']

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

mScarlet with cryptic exon and C-terminal FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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