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Plasmid#198333PurposeExpresses AAGAB-GFP fusion in mammalian cellsDepositorInsertAAGAB (AAGAB Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-TSF-tepsin
Plasmid#196636PurposeExpresses the two Strep/one FLAG (TSF)-tagged human tepsin in mammalian cellsDepositorInserttepsin (TEPSIN Human)
UseTagstwo Strep/one FLAG (TSF)ExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-tepsin
Plasmid#196647PurposeExpression of GAL4 DNA-binding domain (BD)-tepsin fusion proteinDepositorInserttepsin (TEPSIN Human)
UseYeast two-hybrid vectorTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 199 to eliminate int…PromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b-rrt-His8
Plasmid#194088PurposeExpresses Ec86 reverse transcriptase in Escherichia coliDepositorInsertEc86 rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
UseTags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mini-talin P229S (headR13DD P229S)
Plasmid#182995Purposeexpression of minitalin P229S in mammalian cellsDepositorInsertTalin 1 (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationP229SPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
talin1-head P229S
Plasmid#182997Purposeexpression of talin1 head P229S in mammalian cellsDepositorInserttalin1 head P229S (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationP229SPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-HsXRN1_1174-1649-Strep_AH
Plasmid#148901PurposeBacterial Expression of HsXRN1_1174-1649DepositorInsertHsXRN1_1174-1649 (XRN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-DmRoquin-del790-812_AE
Plasmid#148635PurposeMammalian Expression of DmRoquin-del790-812DepositorInsertDmRoquin-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-DmRoquin-del725-755_AE
Plasmid#148633PurposeMammalian Expression of DmRoquin-del725-755DepositorInsertDmRoquin-del725-755 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HseIF1_AC
Plasmid#148441PurposeMammalian Expression of HseIF1DepositorInsertHseIF1 (ELF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF1_AC
Plasmid#148449PurposeMammalian Expression of HseIF1DepositorInsertHseIF1 (ELF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3-Y10E_X
Plasmid#147966PurposeMammalian Expression of HsNanos3-Y10EDepositorInsertHsNanos3-Y10E (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3_X
Plasmid#147962PurposeMammalian Expression of HsNanos3DepositorInsertHsNanos3 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3-del4-20_X
Plasmid#147963PurposeMammalian Expression of HsNanos3-del4-20DepositorInsertHsNanos3-del4-20 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3-F4A_X
Plasmid#147964PurposeMammalian Expression of HsNanos3-F4ADepositorInsertHsNanos3-F4A (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3-W7E_X
Plasmid#147965PurposeMammalian Expression of HsNanos3-W7EDepositorInsertHsNanos3-W7E (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only