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csd6_13-330_Y133A/pET28b(+) N-tag

Plasmid

#71356

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 133 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_C176A/pET28b(+) N-tag

Plasmid

#71360

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Cysteine 176 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H160A/pET28b(+) N-tag

Plasmid

#71359

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 160 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H155A/pET28b(+) N-tag

Plasmid

#71357

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 155 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y132A/pET28b(+) N-tag

Plasmid

#71355

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 132 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-5x3-hMeCP2-delN256-G273X

Plasmid

#48090

Purpose

Bacterial expression of GST tagged AT-Hook2-G273X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-5x3-hMeCP2-delN256-R270X

Plasmid

#48089

Purpose

Bacterial expression of GST tagged AT-Hook2-R270X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-hMeCP2-G273X-R111G

Plasmid

#48087

Purpose

Mammalian expression of Gal4 tagged MeCP2-G273X-R111G

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

Gal4

Expression

Mammalian

Mutation

R111G mutant MeCP2, expresses AT-hook G273X

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Promoter

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
Pet28a His6-HSPB7 (zebrafish) WT

Plasmid

#108917

Purpose

Express WT Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Use

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR V7

Plasmid

#86856

Purpose

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

Depositor

Michael Mancini, Marco Marcelli

Article

Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

Insert

Androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Alternative splice variant 7 (alteration/deletion…

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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InsRA-eGFP

Plasmid

#79795

Purpose

Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Use

Tags

eGFP extracellular

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pKI_ΩBBMVP16&SRDXWUSm1

Plasmid

#220349

Purpose

Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

Depositor

Tomoko Igawa

Article

Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

Inserts

BABY BOOM (BBM Mustard Weed)
WUSCHEL (WUS Mustard Weed)

Use

Tags

Superman Repression Domain X (SRDX) and VP16 tran…

Expression

Plant

Mutation

two amino acid substitution in WUS-box: L255A L25…

Promoter

CaMV 35S and RPS5A

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/td5StayGold(c4)=GianCreg

Plasmid

#212022

Purpose

For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GianCreg (GOLGB1 Human)

Use

Tags

td5StayGold(c4)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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