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Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R2
Plasmid#161494PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R2 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R3
Plasmid#161495PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R3 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R4
Plasmid#161496PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R4 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD163
Plasmid#160935PurposeIn vivo mRNA display construct for mCherryDepositorInsertMCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM03
Plasmid#135417PurposeExpression of Hygromycin resistance from Spizellomyces Hsp70 promoterDepositorInsertSpunHsp70pr
UseTagsExpressionBacterial and YeastMutationPromoterHsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_001_026_096
Plasmid#134402PurposepYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CCG 316
Plasmid#131166PurposeFor ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-BacA-21222-FLP-3xFLAG
Plasmid#109165PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFlp
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM002
Plasmid#122936PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML043C (Marked)
UseTagsExpressionYeastMutationYML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATACPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM001
Plasmid#122935PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC005
Plasmid#122939PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML071C (Marked)
UseTagsExpressionYeastMutationYML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGCPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_207
Plasmid#122938PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML070W (Marked)
UseTagsExpressionYeastMutationYML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_243
Plasmid#122940PurposeExpression of the marked mRNA under the control of insertional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoterPyml029w-tetO(2)Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only