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Plasmid#200681PurposeModular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
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TFORF1470
Plasmid#143282PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX3 (TOX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKGAPDH in pLJM60
Plasmid#154217PurposeLentiviral vector for stable expression of FLAG-TK-GAPDHDepositorInsertGAPDH
UseLentiviralTagsFLAGExpressionMammalianMutationcDNA has been codon optimized for human cell expr…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-PRSx8-AstR-p2A-GFP
Plasmid#159632PurposeLentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoterDepositorInsertAstR (AstA-R1 Fly)
UseLentiviralTagsGFP and P2AExpressionMammalianMutationPromoterPRSx8Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Blast
Plasmid#135682PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2883
Plasmid#144255PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBHLHA9 (BHLHA9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP
Plasmid#162033PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinCS
Plasmid#200309PurposeLentivirus vector expressing vinculin with FRET conformational sensorDepositorInsertVinculinCS
UseLentiviralTagsExpressionMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
Plasmid#194755PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1-P2A-Hygro_Barcode
Plasmid#170221PurposeBarcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xBE3-P2A-Puro
Plasmid#110870PurposeLentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cellsDepositorInsertxCas9(3.7)-BE3
UseLentiviralTagsExpressionMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc
Plasmid#193347PurposetetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc)DepositorUseLentiviralTagsExpressionMammalianMutationSox2A61V is a highly cooperative point mutant of …PromoterTetO mini CMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mouse Gfi1-GFP
Plasmid#91892Purpose3rd generation lentiviral expression of mouse Gfi1DepositorInsertGfi1 (Gfi1 Mouse)
UseLentiviralTagsIRES-EGFPExpressionMammalianMutationPromoterUnknownAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 TagRFP-T LGALS3 R186S
Plasmid#202428PurposeExpression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsTagRFPExpressionMutationR186SPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXIN GFP
Plasmid#132935PurposeMLV-based transfer vector expressing GFP and neomycin resistance proteinDepositorInsertGFP
UseLentiviral, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LeGO-G
Plasmid#27347DepositorInsertU6 promoter, SFFV promoter, eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR'CMVGFP_hRIF1(1355-2446)IREShygro
Plasmid#23136DepositorInsertRIF1 deletion construct (RIF1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast
Plasmid#44017DepositorInsertsUseLentiviralTagsFrb, HA, Hu Frb x2(WT Frb+codon Wobble Frb), V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only