-
Plasmid#223452Purposemgl-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmgl-3 (mgl-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorInsertHiBIT-WDR5 (WDR5 Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY86
Plasmid#191079Purposegcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-25 (gcy-25 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
-
pSN34
Plasmid#100119Purposeexpresses a chimeric OCT4-activator fusionDepositorInsertvexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (POU5F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Mouse, Glycine max)
UseTagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-4T-1 3xFlag-ERK2
Plasmid#47573PurposeBacterial expression plasmid for GST-3xFlag-tagged ERK2DepositorInsertERK2 (Mapk1 Rat)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Brn2.WPRE
Plasmid#67294PurposeInduce human iN conversion from fibroblastDepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM2-FRB-CFP
Plasmid#67518PurposeFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)DepositorInsertmTOR (MTOR Human)
UseTagsCFP, HA, and PM tethering sequence from human Lyn…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorInsertJNK1a1 (MAPK8 Human)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE
Plasmid#67295PurposeInduce human iN conversion from fibroblastDepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
ETV7_A86D_pet28a
Plasmid#131657PurposeBacterial expression of ETS gene with A86D mutation for solubilityDepositorInsertETV7 A86D (ETV7 Human)
UseTags6xHisExpressionBacterialMutationAlanine 86 to Aspartic AcidPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pRB30-GFP
Plasmid#165481PurposepTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and HisDepositorTypeEmpty backboneUseTagsTEV-GFP-FLAG-10x HisExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
ELK3_pet28a
Plasmid#131651PurposeBacterial expression of ETS gene ELK3DepositorInsertELK3 (ELK3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-ADRβ2 Control Plasmid
Plasmid#101123PurposeExpression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cellsDepositorInsertBeta-2-Adrenergic Receptor (ADRB2 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits