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pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

Plasmid

#207171

Purpose

expression plasmid for Gal4DBD-HIF-CAD.myc expression

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD Myc tag linker

Use

Tags

2xMyc tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

Plasmid

#207172

Purpose

expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD GalO linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Lneg-INTR-GFP

Plasmid

#208902

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

Depositor

Christian Gruber

Article

Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

Insert

INTR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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Drosophila Wdr20-V5

Plasmid

#217622

Purpose

Mammalian expression of V5 tagged Drosophila Wdr20

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

WDR20

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD101

Plasmid

#212925

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 Prg4 promoter-luciferase

Plasmid

#195034

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#208605

Purpose

Expressing CheRiff_EGFP under CMV promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRiff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD140

Plasmid

#212927

Purpose

Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD099

Plasmid

#212923

Purpose

A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD113

Plasmid

#212926

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD100

Plasmid

#212924

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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α-synuclein-3H

Plasmid

#205715

Purpose

Mammalian expression of alpha-synuclein-3H

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-αS-2

Plasmid

#205706

Purpose

Mammalian expression of alpha-synuclein tandem dimer

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein tandem dimer (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l3-A'

Plasmid

#209118

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l3-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-G'

Plasmid

#209122

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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